Scientist, Protein Biochemistry

Elevian is seeking a dedicated, innovative, and motivated scientist with extensive hands-on experience in protein expression in mammalian and/or in other systems, protein purification, and protein characterization including the development and execution of cellular, biochemical, and biophysical assays. The successful candidate will be an important member of Elevian’s R&D team and contribute to the discovery and development of recombinant protein and antibody therapeutics for stroke and other degenerative diseases.

Primary Responsibilities:

  • Produce protein in mammalian cells and/or in prokaryotic systems both internally and in collaboration with CROs and CDMOs

  • Develop scalable expression and purification processes

  • Develop and implement assays to characterize protein activity and function in vitro

  • Characterize basic biochemical, biophysical, and functional activity properties of recombinant proteins and antibodies in vitro and support qualification of candidates for in vitro and in vivo efficacy studies

  • Support antibody discovery and development efforts

  • Design and assemble vectors for recombinant protein expression

  • Design and engineer novel protein variants of Elevian’s therapeutic candidates

  • Independently design and execute experiments, including data analysis, communication of results and proposals for next steps.

  • Present results, both internally and externally.

 

Education, Behavioral Competencies, Skills, and Qualifications

  • PhD in Biochemistry, Biophysics, or other related disciplines with 2 - 4 years of relevant industry or academic experience

  • Strong background in protein structural biology and biochemical and biophysical methods of protein characterization

  • Experience in purification of tagged and untagged proteins derived from various expression systems (mammalian, insect and bacterial).

  • Expertise in standard protein chromatography techniques on an AKTA FPLC (or similar) systems including affinity chromatography, IEX, SEC, and reverse-phase etc.

  • Experience with analyzing proteins (SE-HPLC, SDS-PAGE, Western Blots, and endotoxin assays)

  • A deep understanding of modern cloning approaches, rapid construct design, basic bioinformatics, and tagging strategies for the expression and purification of protein

  • Demonstrated ability to implement a variety of construct designs, different expression strategies, and use of multiple expression systems

  • Experience with mammalian cell culture and aseptic techniques

  • Excellent team working skills and adaptability

  • Ability to work independently, design experiments, follow and adapt protocols, conduct, interpret, and optimize experimental studies is a must.

  • The candidate should have excellent verbal and written communication skills. Clear scientific presentation skills during project and team meetings are required.

  • Must have excellent organization skills. The candidate is expected to document all aspects of experiments performed and be able to conduct independent research to collect and archive all necessary information from the literature.

 

Preferred Qualifications

  • Experience with biochemical/biophysical assays such as SPR, BLI, AUC, ITC, etc. is highly desired.

  • Experience with antibody purification and characterization is highly desired

  • Experience with transient and stable mammalian (CHO and HEK) protein expression

  • Experience with cell-based activity-reporter assays

Please reach out to us at careers@elevian.com to share your resume and learn more. Please indicate the position you wish to be considered for in the subject line.

Sharon Reaves